Introduction
Cartilage has two general roles : during development, cartilage represents a necessary but transitory step, shaping a model of the skeleton. In the adult organism, cartilage is a permanent tissue of outstanding biomechanical properties, providing joints with elasticity and high mechanical stability. However, articular cartilage is not vascularized and presents poor intrinsic healing potential. Consequently, traumatic and degenerative lesions of articular cartilage eventually progress to osteoarthritis, a worldwide leading source of disability. Regarding cartilage repair, common surgical treatments (microfracture, mosaicplasty) are not satisfactory, since often leading to the production of fibrocartilage which does not present the biomechanical properties of articular cartilage. Joint replacement is a short-term therapy because knee prostheses have limited life spans, which is a problem for young people. In this context, the regeneration of cartilage requires cell therapy and tissue engineering techniques. In order to reconstruct good-quality cartilage, it is important to have a good understanding of the cellular and molecular events which control chondrocyte differentiation and cartilage development.
Interestingly, many cellular and molecular events occurring during fetal cartilage formation illegitimately take place in osteoarthritic cartilage (figure 1).
Figure 1 : Illustration of our approach integrating diverse aspects of the chondrocyte physiology. Several molecular and cellular events occurring during endochondral ossification illegitimately take place in osteoarthritic articular cartilage in adult life (for instance matrix degradation). Therefore the understanding of all these events helps to set up new strategies for cell therapy and tissue engineering of cartilage
Themes
Our group takes advantage of its long-term studies on chondrocyte differentiation and cartilage development to set up new protocols to repair cartilage, in collaboration with hospitals and industries. More specifically, we analyze :
- The signaling events triggered by bone morphogenetic protein (BMP)-2 and transforming growth factor (TGF)-β1 (E. Aubert-Foucher)
- The pathways of mechanotransduction (E. Perrier-Groult)
- The signaling pathways under the control of hypoxia, physiological condition of normal cartilage (J. Lafont)
- The influence of diverse tridimensional matrices and biomaterials on the chondrocyte phenotype (E. Perrier-Groult)
- The pathophysiology and regeneration of the pulpodental complex (J-C. Farges)
- The characteristics of diverse sources of mesenchymal stem cells and their potential to differentiate into skeletal lineages (F. Mallein-Gerin)
Keywords : cartilage, chondrocyte, mechanotransduction, BMP-2 signaling, hypoxia, ingeneering , dental-pulp complex, stem cells
Members : ROAD group
Publications : Publications ROAD group
Contact
ROAD group (osteoarticular and dental research)
Laboratoire de Biologie Tissulaire et Ingénierie thérapeutique – CNRS UMR 5305 – University Claude Bernard Lyon 1
Institut de Biologie et Chimie des Protéines
7, passage du Vercors 69367 LYON CEDEX 07
FRANCE
E-mail : f.mallein-gerin@ibcp.fr
Tel : +33 (0)4-37-65-29-19
Fax : +33 (0)4-72-72-26-04
