{"id":1923,"date":"2017-07-21T13:53:11","date_gmt":"2017-07-21T11:53:11","guid":{"rendered":"http:\/\/lbti.ibcp.fr\/?page_id=1923"},"modified":"2023-06-27T13:39:09","modified_gmt":"2023-06-27T11:39:09","slug":"physiopathologie-et-regeneration-du-complexe-pulpodentinaire","status":"publish","type":"page","link":"https:\/\/lbti.ibcp.fr\/?page_id=1923","title":{"rendered":"Innovative antibacterial hydrogels for dental pulp regeneration"},"content":{"rendered":"

Project leader<\/strong><\/em>\u00a0 : M. Ducret<\/a><\/strong><\/span><\/h4>\n

Participant\u00a0 <\/strong><\/em>: M. Bekhouche, J-C Farges, M. Pasdeloup, M. L\u00e9v\u00eaque<\/span><\/p>\n

\n

Infections of teeth are a major concern of public health worldwide. Two strategies are proposed to regenerate dental pulp: a \u00ab cell-based strategy \u00bb uses exogenous cells and a biomaterial (i), and a \u00ab cell-free strategy \u00bb allows host cells to colonise a biomaterial to reconstruct a new dental pulp (ii).<\/span><\/p>\n

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Figure 1 : Illustration of two strategies for dental pulp reconstruction<\/em>.<\/span><\/p>\n

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Figure 2 : Illustration of different axes of the project ENDONANOBIOTIC<\/em><\/span><\/p>\n

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Selected publications :\u00a0<\/strong><\/em><\/span><\/p>\n

\n

-Bekhouche M, Bolon M, Charriaud F, Lamrayah M, Da Costa D, Primard C, Costantini A, Pasdeloup M, Gobert S, Mallein-Gerin F, Verrier B, Ducret M, Farges JC.<\/span> Development of an antibacterial nanocomposite hydrogel for human dental pulp engineering. <\/a>J Mater Chem B.<\/strong> 2020 Sep 23;8(36):8422-8432. doi: 10.1039\/d0tb00989j.<\/span> PMID: 32804177<\/span><\/span><\/p>\n

– <\/b>Ducret M, Farges JC, Pasdeloup M, Perrier-Groult E, Mueller A, Mallein-Gerin F, Fabre H. Phenotypic Identification of Dental Pulp Mesenchymal Stem\/Stromal Cells Subpopulations with Multiparametric Flow Cytometry.<\/a><\/p>\n

Methods Mol Biol<\/span>.<\/strong> 2019;1922:77-90. doi: 10.1007\/978-1-4939-9012-2_8.<\/p>\n<\/div>\n

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– Ducret M, Montembault A, Josse J, Pasdeloup M, Celle A, Benchrih R, Mallein-Gerin<\/b> F, Alliot-Licht B, David L, Farges JC. Design and characterization of a chitosan-enriched fibrin hydrogel for human dental pulp regeneration.\u00a0<\/a>Dent Mater<\/span>.<\/strong> 2019 Apr;35(4):523-533. doi: 10.1016\/j.dental.2019.01.018. Epub 2019 Jan 31.<\/p>\n<\/div>\n

– <\/strong>Ducret M, Fabre H, Celle A, Mallein-Gerin F, Perrier-Groult E, Alliot-Licht B, Farges JC.<\/span> Current challenges in human tooth revitalization.<\/a> Biomed Mater Eng.<\/em><\/strong> 2017;28(s1):S159-S168. PMID: 28372291. doi: 10.3233\/BME-171637.<\/span><\/p>\n

– <\/strong>Ducret M, Fabre H, Degoul O, Atzeni G, McGuckin C, Forraz N, Mallein-Gerin F, Perrier-Groult E, Alliot-Licht B, Farges JC.<\/span> Immunophenotyping Reveals the Diversity of Human Dental Pulp Mesenchymal Stromal Cells In vivo<\/em> and Their Evolution upon In vitro<\/em> Amplification.<\/u><\/a> Front Physiol<\/strong>.<\/strong> 2016 Nov 8;7:512. eCollection 2016. PMID: 27877132. doi: 10.3389\/fphys.2016.00512<\/span><\/p>\n

– <\/strong>Park S-H, Ye L, Love RM, Farges J-C, Yumoto H.<\/span>\u00a0Inflammation\u00a0of the\u00a0Dental Pulp.<\/a> Mediat Inflamm<\/strong> 2015; Article ID 980196. doi: org\/10.1155\/2015\/980196.<\/span><\/p>\n

– Ducret M, Fabre H, Degoul O, Atzeni G, Mcguckin C, Forraz N, Alliot-Licht B, Mallein-Gerin F, Perrier-Groult E, Farges J-C.<\/span> Manufacturing of dental pulp cell-based products from human third molars\u00a0: current strategies and future investigations.<\/a> Front Physiol<\/strong> 2015\u00a0;6:213. doi:10.3389\/fphys.2015.00213.<\/span><\/p>\n

– Ducret M, Fabre H, Farges J-C, Degoul O, Atzeni G, Mcguckin C, Forraz N, Mallein-Gerin F, Perrier-Groult E.<\/span> Production of human dental pulp cells with a medicinal manufacturing approach.<\/a> J Endod<\/strong> 2015\u00a0; 41(9):1492-9. doi\u00a0: 10.1016\/j.joen.2015.05.017.<\/span><\/p>\n

– Farges J-C, Bellanger A, Ducret M, Aubert-Foucher E, Richard B, Alliot-Licht B, Bleicher F, Carrouel F.<\/span> Human odontoblast-like cells produce nitric oxide with antibacterial activity upon TLR2 activation<\/a>. Front Physiol<\/strong> 2015\u00a0;6:185. doi\u00a0: 10.3389\/fphys.2015.00185.<\/span><\/p>\n

– Gaudin A, Renard E, Hill M, Bouchet-Delbos L, Bienvenu-Louvet G,\u00a0Farges J-C, Cuturi M-C, Alliot-Licht B.<\/span> Phenotypic analysis of immunocompetent cells in healthy human\u00a0dental pulp.<\/a> J Endod <\/strong>2015\u00a0;41(5):621-7. doi\u00a0: 10.1016\/j.joen.2015.01.005.<\/span><\/p>\n

– Bonnamain V, Thinard R, Sergent-Tanguy S, Huet P, Bienvenu G, Naveilhan P, Farges J-C, Alliot-Licht B.<\/span>\u00a0Human\u00a0dental pulp\u00a0stem cells\u00a0cultured\u00a0in\u00a0serum-free\u00a0supplemented\u00a0medium.<\/a> Front Physiol<\/strong> 2013\u00a0;4:357. doi:10.3389\/fphys.2013.00357.<\/span><\/p>\n

– Farges J-C, Alliot-Licht B, Baudouin C, Msika P, Bleicher F, Carrouel F.<\/span>\u00a0Odontoblast\u00a0control\u00a0of\u00a0dental\u00a0pulp\u00a0inflammation\u00a0triggered\u00a0by\u00a0cariogenic\u00a0bacteria.<\/a> Front Physiol<\/strong> 2013\u00a0;4:326. doi\u00a0: 10.3389\/fphys.2013.00326.<\/span><\/p>\n

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Collaborations :\u00a0<\/strong><\/em><\/span><\/p>\n

-Pr Colin McGUCKIN & Dr Nico FORRAZ, CTI-BIOTECH, Meyzieu.<\/p>\n

-Dr Audrey AUSSEL, Dr Nicolas L\u2019heureux, Dr Olivia K\u00e9rour\u00e9dan, Laboratoire Biotiss, Bordeaux.<\/p>\n","protected":false},"excerpt":{"rendered":"

Project leader\u00a0 : M. Ducret Participant\u00a0 : M. Bekhouche, J-C Farges, M. Pasdeloup, M. L\u00e9v\u00eaque Infections of teeth are a major concern of public health worldwide. Two strategies are proposed to regenerate dental pulp: a \u00ab cell-based strategy \u00bb uses exogenous cells and a biomaterial (i), and a \u00ab cell-free strategy \u00bb allows host cells<\/p>\n","protected":false},"author":1,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_acf_changed":false,"footnotes":""},"class_list":["post-1923","page","type-page","status-publish","hentry"],"acf":[],"_links":{"self":[{"href":"https:\/\/lbti.ibcp.fr\/index.php?rest_route=\/wp\/v2\/pages\/1923","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/lbti.ibcp.fr\/index.php?rest_route=\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/lbti.ibcp.fr\/index.php?rest_route=\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/lbti.ibcp.fr\/index.php?rest_route=\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/lbti.ibcp.fr\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=1923"}],"version-history":[{"count":16,"href":"https:\/\/lbti.ibcp.fr\/index.php?rest_route=\/wp\/v2\/pages\/1923\/revisions"}],"predecessor-version":[{"id":5248,"href":"https:\/\/lbti.ibcp.fr\/index.php?rest_route=\/wp\/v2\/pages\/1923\/revisions\/5248"}],"wp:attachment":[{"href":"https:\/\/lbti.ibcp.fr\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=1923"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}