Evaluation of inflammation pattern of a buccal patch for vaccine delivery
Administrative supervisor: Dr Claire MONGE
Scientific supervisor: Anne-Lise PARIS
« Colloidal vectors and tissue transport »
Most of infectious diseases use mucosal tissues as portal of entry. Yet, most of traditional injected vaccines are poor inducers of mucosal immunity and are then less effective against infections at mucosal sites. Thus, mucosal vaccines would be the more appropriate prevention against mucosal infections, as it can induce local protective immunity at mucosal sites as a first line of defence as well as effective induction of systemic immunity. Among mucosal administration routes, buccal route, and in particular sublingual region, is an interesting, needle-free and efficient way of immunization since the epithelium of the sublingual mucosa is particularly thin and allows easy penetration of antigens. The common sublingual administration of antigen is currently performed by liquid deposition under the tongue but the induced immune response is moderate – although promising- due to the low residence time of antigen at the mucosal site and rapid clearance by saliva.
In our group, we develop mucoadhesive patches made of natural polymers by the Layer-by-layer technology. To evaluate the potential of these patches to be used as vaccine delivery systems, we are looking for a motivated student to perform the evaluation of the immune response after patch application both in vitro and in vivo. The project includes histology, cell culture, flow cytometry, confocal microscopy as well as patch production.
The successful candidate will work in a highly transdisciplinary team (biology, chemistry, biotechnology).
LbL technology: Monge et al, Advanced Healthcare Materials, 2015
Polymeric membrane for bone engineering: Caridade et al, Acta Biomaterialia, 2015
Membrane degradation: Cardoso et al, Biomacromolecules, 2016